Wayne Stollings wrote:
Odd too that according to PETA and HSUS letters dated this spring, the SNAP-25 test is not validated for use by the U.S. FDA either.http://iccvam.niehs.nih.gov/methods/bio ... Abotox.pdf
SUBJECT: IDEXX Transition to the New SNAP. Beta-lactam Test Kit
Below is the text of a letter received by FDA from Larry Chaney, Ph.D., Dairy
Business Manager, IDEXX Laboratories, Inc.
"Based on FDA's approval of the transition to New SNAP. Beta-lactam test kits,
IDEXX Laboratories, Inc. will manage the inventory of the remaining SNAP
Beta-lactam product based on the customer's demand in an expeditious manner. As
you may be aware, the product is available in both 20 test and 100 test kit
quantities. While we will attempt to completely deplete the inventory of
current product, it may be necessary to sell New SNAP. Beta-lactam based on
customer's test kit quantity demand, creating a limited amount of time that the
two products will be sold.
No it's not validated for the replacement of the LD50 tests in mice.
Division of Bacteriology, National Institute for Biological Standards and Control, South Mimms, Hertfordshire, UK.
Bacterial neurotoxins are now being used routinely for the treatment of neuromuscular conditions. Alternative assays to replace or to complement in vivo bioassay methods for assessment of the safety and potency of these botulinum neurotoxin-based therapeutic products are urgently needed. Advances made in understanding the mode of action of clostridial neurotoxins have provided the basis for the development of alternative mechanism-based assay methods. Thus, the identification of SNAP-25 (synaptosomal-associated protein of molecular mass 25 kDa) as the intracellular protein target which is selectively cleaved during poisoning by botulinum neurotoxin type A (BoNT/A) has enabled the development of a functional in vitro assay for this toxin. Using recombinant DNA methods, a segment of SNAP-25 (aa residues 134-206) spanning the toxin cleavage site was prepared as a fusion protein to the maltose-binding protein in Escherichia coli. The fusion protein was purified by affinity chromatography and the fragment isolated after cleavage with Factor Xa. Targeted antibodies specific for the N and C termini of SNAP-25, as well as the toxin cleavage site, were prepared and used in an immunoassay to demonstrate BoNT/A endopeptidase activity towards recombinant SNAP-25 substrates. The reaction required low concentrations of reducing agents which were inhibitory at higher concentrations as were metal chelators and some inhibitors of metallopeptidases. The endopeptidase assay has proved to be more sensitive than the mouse bioassay for detection of toxin in therapeutic preparations. A good correlation with results obtained in the in vivo bioassay (r = 0.95, n = 23) was demonstrated. The endopeptidase assay described here may provide a suitable replacement assay for the estimation of the potency of type A toxin in therapeutic preparations.
PMID: 9353935 [PubMed - indexed for MEDLINE]
http://www.ncbi.nlm.nih.gov/entrez/quer ... t=Abstract
I am not sure your debating tactics are designed to discuss rather than "I told you so" and not very healthy.
Is this going to turn into a google search test?
It is easy to select sections of text and find something to contradict it and the posting "copy right" rules helps this.
When faced with a test that works and is an alternative to painfully killing animals then I would expect any decent person would want it used.
Seems I was wrong. some people argue the toss for animal testing for what seems not real reason.